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This was evaporated, redissolved in water and finally reconstituted in 90:10 water/methanol to give a volume of 0.5 ml.
Water from 3️⃣ an ELGA PURELAB Chorus 1 was used throughout this work.
The absence of trace organic contaminants in the water is critical 3️⃣ as water is used repeatedly in the sample preparation: in the hydrochloric acid used to acidify the sample, to condition 3️⃣ the SPE cartridge and to rinse it twice, to enhance the removal of acetonitrile
Samples were taken from the inlets to 3️⃣ wastewater treatment plants in locations where sporting events of various types were taking place, before and during the events.
It was 3️⃣ found to be essential to freeze the samples prior to analysis.
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